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Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing <t>PCNA</t> and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.
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Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing <t>PCNA</t> and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.
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Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing <t>PCNA</t> and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.
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Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing <t>PCNA</t> and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.
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Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing PCNA and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Spatially resolved multi-omics reveals the renal cortex-metabolic reprogramming of Shenhua Tablet for intervention on IgA nephropathy.

doi: 10.1016/j.phymed.2025.156742

Figure Lengend Snippet: Fig. 2. SHT protects from renal dysfunction, mesangial cell proliferation and inflammatory responses in IgAN rats. (A) The 24 h Upro, Scr and BUN levels in the experimental groups (n = 8 in each group). (B) Representative images showing PCNA and PAS staining and semi-quantitative analysis of mesangial cell pro liferation in the experimental groups (n = 6 in each group). Scale bars, 20 μm. (C) Serum IL-6, IL-1β and TNF-α levels in the experimental groups (n = 8 in each group). (D) Immunohistochemical images and semi-quantitative analysis of OX8 and CD68 expression in the experimental groups. (n = 6 in each group). All sta tistical data are presented as mean ± SD and analyzed by ordinary one-way ANOVA followed by Tukey’s multiple comparison test. Comparative analysis, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.

Article Snippet: The corresponding stain reagent was PAS Stain Kit (Solarbio, G1281), whereas the antibodies used included PCNA rabbit polyclonal antibody (1:200 dilution, Proteintech, 10,205–2-AP), Rabbit Anti-ODC1 antibody (1:200 dilution, Bioss, bs1294R), Col4 Polyclonal antibody (1:300 dilution, Bioss, bsm56208R), CD68 rabbit polyclonal antibody (1:200 dilution, Servicebio, GB113109), OX8 antibodies (1:200 dilution, boster, A02236–1), and HRP-conjugated goat anti-rabbit IgG (H + l) (1:200 dilution, ServiceBio, GB23303).

Techniques: Staining, Immunohistochemical staining, Expressing, Comparison